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A study of SecA : the motor of the bacterial secretion system by site-directed spin labeling and EPR
(University of Missouri--Columbia, 2008)
For more than 3 decades, the biochemical details of the Sec system of protein export have been teased out of Escherichia coli. Through these efforts we know that SecA, the ATPase of the Sec system, interacts with several ...
Identification of novel breast carcinoma and melanoma avid peptides for imaging
(University of Missouri--Columbia, 2009)
During the last decade, peptides and their derivatives have been employed as effective cancer imaging agents. We aimed to identify new peptides probes specific for melanoma and breast carcinoma by phage display technology. ...
Structural studies of glyceraldehyde-3-phosphate dehydrogenase complexes and the E. coli PutA DNA binding domain
(University of Missouri--Columbia, 2006)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that catalyzes the formation of 1,3-bisphosphoglycerate from glyceraldehyde-3-phosphate. We have solved a high-resolution (1.75 Å) structure of a ...
In vitro characterization of Nudt16 orthologs by sequence and function provide evidence supporting a nuclear RNA degradation pathway
(University of Missouri--Columbia, 2009)
Nudt16, previously called X29, is known to be a metal-dependent decapping enzyme expressed in mammals and Xenopus . Nudt16 has been proposed to be the catalytic component of a previously unknown nuclear RNA degradation ...
Structural studies of PMM/PGM from Pseudomonas aeruginosa
(University of Missouri--Columbia, 2005)
The human pathogen Pseudomonas aeruginosa expresses a variety of cell surface polysaccharides, including alginate, lipopolysaccharide (LPS), and rhamnolipid. P. aeruginosa is the primary cause of chronic lung infections ...
Kinase-interacting FHA domain of kinase associated protein phosphatase : phosphopeptide interactions and NMR-detected dynamics
(University of Missouri--Columbia, 2007)
rigidifies KI-FHA at the binding site and remote sites across the [beta-] sandwich. Peptide binding increases flexibility around the periphery of the binding site, perhaps relieving strain from the peptide association....
Merging technology development of trapped ion mobility spectrometry quadrupole time-of-flight mass spectrometry with biological investigation of Medicago truncatularoot exudates
(University of Missouri--Columbia, 2020)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] "New commercial ion mobility instrumentation has the potential to improve current analytical techniques, but developments need to be applicable to ...
Physiologic and metabolic interactions in the soybean/bradyrhizobium japonicum symbiosis
(University of Missouri--Columbia, 2006)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] The agronomically vital, nitrogen-fixing symbioses between soybean and the soil bacterium Bradyrhizobium japonicum is a model system for the study of ...
Structural and functional studies of proline catabolic enzymes
(University of Missouri--Columbia, 2007)
Catabolism is the oxidation of organic nutrients into simple end molecules to extract energy. For proline, the catabolic pathway involves two enzymes, Lproline dehydrogenase (PRODH, EC 1.5.99.8) and L-[delta]¹-pyrroline- ...
Viral RNA structural equilibria and their interactions with host proteins
(University of Missouri--Columbia, 2019)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Understanding viral RNA structure and how it functions is crucial in elucidating new drug targets. There are many kinds of viruses that utilize RNA ...
A custom oligonucleotide microarray analysis as a tool for dissecting soybean-bradyrhizobium japonicum nodule senescence
(University of Missouri--Columbia, 2007)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Soybean root nodules are symbiotic organs housing Bradyrhizobium japonicum, a soil bacterium that reduces atmospheric nitrogen to ammonium. The bacterium ...
Protein phosphorylation regulation in Arabidopsis
(University of Missouri--Columbia, 2006)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Protein phosphorylation and dephosphorylation are fundamental mechanisms involved in many cell-signaling events. Coordinated actions of protein kinases ...
Regulation of Nrf2 by a keap1-dependent E3 ubiquitin ligase
(University of Missouri--Columbia, 2007)
Oxidative damage to biological macromolecules has been implicated in diverse pathophysiological processes, including cancer, neurodegeneration, aging and cardiovascular disease. The Nrf2 transcription factor regulates ...
Understanding Arabidopsis ion homeostasis in the post-genomic era : assigning function to two proteins involved in iron metabolism
(University of Missouri--Columbia, 2006)
Two projects studying different aspects of iron deficiency in the model plant Arabidopsis thaliana are detailed here. The first project describes the isolation and characterization of the Arabidopsis frd4-1 and frd4-2 ...